primer n. 1.初级读本,初学书;初阶,入门(书);(尤指宗教改革前的)小祷告书。 2.【印刷】10点或18点活字的旧称。 great primer 18 点铅字。 long primer 10 点铅字。 primer2 n. 1.装填火药者;装雷管者;起动油泵者。 2.【机械工程】初给器;发火极;发火药;导火线;爆管,雷管;火帽;底火。 3.底漆,首涂油。 4.【生物化学】引体。
Among 70 arbitrary primers , 13 primers could get enough amplified bands for all strains 这13个引物对11株供试菌株均能扩增出清晰的谱带。
The procedure of ddrt - pcr applicable for silver staining was optimized by adjusting the amount of several critical reagents , including total rna , anchor primer , arbitrary primer , cdna and dntp 通过调整ddrt - pcr中总rna 、锚定引物、随机引物、 cdna和dntp等关键试剂的用量,优化了适用于银染检测的ddrt - pcr方法。
Among 54 arbitrary primers tested , only 9 primers could generate clear and reproducible fragments in 32 species belonging to 12 genera of the family gryllidae ; 9 primers in 15 species belonging to 7 genera of the family trigonidiidae ; 12 primers in 3 species belonging to the genus oecanthus of the family oecanthidae ; 12 primers in 4 species belonging to 3 genera of the family eneopteridae ; 13 primers in 1 species belonging to the genus homoeogryllus of the family phalangopsidae ; 13 primers in 1 species belonging to the genus sclerogryllus of the family sclerogryllidae ; and 13 primers in 1 species belonging to the genus eulandrevus of the family gryllomorphidae 本研究经用54种随机引物对57种蟋蟀的rapd扩增分析得出以下结论: 1 )采用rapd技术,首次较系统地对中国蟋蟀总科7科26属共57种蟋蟀进行了系统学研究。在所试用的54种随机引物中筛选出可扩增出清晰可辨且稳定的rapd图谱的引物分别为:蟋蟀科9种、岭蟋科9种、树蟋科12种、结蟋科12种、蛛蟋科13种、铁蟋科13种、貌蟋科13种。
Rapd ( random amplified polymorphic dna ) , which bases on the polymerase chain reaction ( pcr ) , is by far one of the most commonly molecular techniques to uncover dna sequence polymorphisms . the basic priciple of this technique is that an arbitrary primer ( usually lobp oligonudetide ) is used to amplify random segments of dna , and a small number of fragments will be amplified when the primer anneals on each strand over a length range . if sequence variation is present at the priming site , then a fragment may not be amplied , so the dna polymorphic can be detected Rapd (随机扩增多态性dna )技术是二十世纪90年代发展起来的一项dna分子多态性检测技术,它建立于聚合酶链式反应( pcr )技术基础之上,利用随机合成的寡聚核苷酸序列为引物(一般为10个bp ) ,分别与dna的两条单链结合,在dna聚合酶的作用下,对基因组的特定区域进行pcr扩增,其电泳结果为不同大小和数目的dna谱带即rapd图谱,可反映基因组相应区域的dna多态性。
Forty arbitrary primers were used in the analysis of the seven samples , within which five primers produced relatively stable diversity . in total 49 loci were detected by these five primers , including 25 diverse loci ( 51 % ) . pairwise genetic distances were calculated and accordingly cluster analysis was carried out and the genetic dendrogram of the seven samples were generated 在rapd分析中使用了40个随机引物对7个样本进行分析,经重复实验,筛选出了5个多态性较为稳定的引物,测到49个位点,其中25个( 51 )有多态性。